Check that
all the connections of the instrument are proper.
Ensure that
printer is attached to the instrument.
Attach
sample compartment.
Log in the
software.
Instrument
may ask for gathering the configuration, and then click OK.
System
suitability set up will appear.
Go to
checks & select only Abscissa, screen report.
Go to
archive & set archive prefix as required & click OK.
Resolution
Go to
instrument > Scan > Instrument > Advanced.
Keep
Apodization 'none’ & scan speed 0.1 cm/s
Instrument
may ask for collecting new background, then click OK.
Go to instrument
> Validate > system Suitability & click OK.
Instrument
should display ‘ system suitability test pass’.
Scroll the
report from original path for display, click print icon & take print out.
Abscissa
spectrum of polystyrene film will be displayed.
Go to graph
format icon & select the range 1600 to 1580.
Zoomed
spectrum will appear, then collect the continuous curser & label at about
1583 cm-1 & 1589 cm-1, find out %T at these wave numbers & find out the
difference between two %T.
Differences
should be not less than 12%T.
Similarly
zoom between 2840 cm-1 to 2880 cm-1 & label at 2851 cm-1 & 2870 cm-1,
find out %T at these wave numbers & find out difference.
Difference
should be not less than 18%T.
Then adjust
the scale to 4000 to 450 cm-1 & take print outs.
Verification
of wave number scale
Go to Process
> Peak table > take its print.
Peak
(transmission minima) should be observed at the wave numbers as follows, with
in the tolerance limit.
Wave number cm-1
|
Minimum Tolerance
cm-1
|
Maximum tolerance
cm-1
|
3060.0
|
3058.5
|
3061.5
|
2849.5
|
2848.0
|
2851.0
|
1942.9
|
1941.4
|
1944.4
|
1601.2
|
1600.2
|
1602.2
|
1583.0
|
1582.0
|
1584.0
|
1154.5
|
1153.5
|
1155.5
|
1028.3
|
1027.3
|
1029.3
|
Remove
sampling compartment.
Attach the
diffuse reflectance sampling accessory.
Collect the
background with KBr.
Go to set
up > Validation > System suitability.
Set up will
appear.
Go to
checks & select only Abscissa, screen report.
Go to
archive & set archive prefix as required & click OK.
Go to Instrument
> Validate > System suitability.
Click OK.
Instrument
should display System suitability test passes.
Report will
be displayed, click print icon & take print out.
Maintenance
/ Repairs
Whenever
instrument is out of order, then put the tag as Out of Calibration & get it
repaired. After repair calibrate the instrument before use.
Frequency:
Once in
three months.
Attachments
Enclose
following attachments with the calibration report.
-
System
suitability check report for side holder instrument configuration.
-
Abscissa
spectrum of polystyrene film with identified peaks table for wave number
calibration.
-
Abscissa
spectrum of polystyrene film (showing %T at 2851 cm-1, 2870 cm-1, 1583 cm-1,
1589 cm-1).
-
System
suitability check report for diffuse reflectance instrument configuration.
Note: Words in italics stand for instrument tabs.
CALIBRATION REPORT OF FT-IR SPECTROPHOTOMETER
Reference - Date
–
Frequency – Once in three Months. Instrument
No. –
Make –
1.0
Resolution:
Difference Between % T at 2851 cm-1 and 2870 cm-1
|
Difference Between %T at 1583 cm-1 and 1589 cm-1
|
|
Limit
|
NLT 18%
|
NLT 12%
|
Actual
|
2.0
Verification wage Number Scale:
ObservedWave
number cm-1
|
Minimum Tolerance
cm-1
|
Maximum tolerance
cm-1
|
3060.0
|
3058.5
|
3061.5
|
2849.5
|
2848.0
|
2851.0
|
1942.9
|
1941.4
|
1944.4
|
1601.2
|
1600.2
|
1602.2
|
1583.0
|
1582.0
|
1584.0
|
1154.5
|
1153.5
|
1155.5
|
1028.3
|
1027.3
|
1029.3
|
3.0
System
suitability after attachment of diffuse reflectance sampling accessory: Complies
/ Does Not Comply.
4.0
Conclusion:
Calibration is satisfactory / Not satisfactory.
5.0
Next Calibration Due On:
Done By:
Date:
|
Checked By:
Date:
|
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