ASSAY OF ASCORBIC ACID

Reagents Required:

1.0M Sulfuric acid

0.05M Iodine

Procedure

Weigh accurately about 0.1 gm of sample and dissolve in a mixture of 100 ml freshly boiled and cooled water and 25 ml of 1M sulfuric acid. Immediately titrate with 0.05M iodine, using starch as indicator until a persistent blue-violet color is obtained. Each ml of 0.05M iodine is equivalent to 0.008806 gm of ascorbic acid.

Calculation:

% of Ascorbic acid = BR X Actual molarity X 0.008806 X 100

                                                      0.05 X Wt. of Sample

BR = Burette Reading